College of Pharmacy
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MCP-SIL

The Small Instrument Laboratory of the Department of Medicinal Chemistry and Pharmacognosy

SIL photo
SIL Photo

MCP-SIL – The Small Instrument Laboratory of the Department of Medicinal Chemistry and Pharmacognosy

Description

The Small Instrument Laboratory (420 COP) is equipped to perform routine type spectroscopic and chromatographic analyses. Spectroscopic equipment available includes UV/VIS, fluorescence, and ATR-FT-IR instruments. Furthermore, the laboratory offers high-speed, temperature and pressure controlled centrifugal vacuum evaporation for the preparation of chemical and biological samples in the <1 to 500 ml range. For the preparative separation of natural products and other (complex) mixtures countercurrent (HSCCC, DCCC) and flash (LPLC) chromatography methods are available, including sample handling for 96-well plates. The chromatography section of the laboratory is supplemented by analytical HPLC and GC equipment.

Rules for Use

  1. All users are required to adhere to the following set of rules when using the facility:
  2. Two Responsible Persons from the Department are the primary contact for each type of instrument. They are in charge of maintaining the equipment and training the users. Two Department members, who report to the faculty in charge of the facility, share the overall responsibility of maintaining the facility.
    • Each responsible team makes sure that in times of absence one person is available.
  3. Before using any equipment, it is mandatory that a user has received personal training from one of the Responsible Persons.
    • The training is offered on a day-to-day basis or in groups, as needed.
    • Training is documented by the Responsible Persons, who also supply standard operation manuals.
  4. Keeping and signing the logbook is mandatory.
  5. Users are still responsible for operating the instruments properly.
  6. In case of problems, malfunction or breakdown, the Responsible Persons must be consulted.
  7. The SIL is an analytical facility - keep it tidy!
  8. Access to some sensitive equipment will be granted on a personal basis only. This requires to sign-in in the Departmental Office prior to use, and to sign-out after completion of the experiments.
  9. No eating and drinking in this laboratory.
  10. No borrowing of equipment from this laboratory.
  11. Keep the door locked at all times.
  12. Maintain the safety precautions applicable, like to any laboratory. Note that this laboratory is not a biocontainment or radiation safety level facility.

Equipment

SpeedVac Concentrator Thermo Forma SPD III V System

  • SPD111V Concentrator
  • SPUVS400 Refrigerated Trap
  • SPCPOF110 Vacuum Source
  • 40x1.5 ml rotor

SpeedVac Concentrator Thermo Forma SC 250 EXP System

  • SC250 EXP Concentrator
  • RVT4104 Vacuum Source
  • 28x56, 16x125, 13x100 mm rotor blocks

High-Speed Countercurrent Chromatography (HSCCC) system

  • PharmaTech CCC-1000 chromatograph
  • Triple coils with total volume of 120 ml
  • SSI prep HPLC pump Model III
  • 6-port valve injection system
  • fraction collector with test tube and 96-well plate capabilities

Universal UV detection system

  • ShimadzuSPD-10AVP dual-wavelength detector with preparative and analytical flow cells
  • Cole–Parmer model 80807 paperless chart recorder and digitizer with 3.5'' floppy drive and 3-channel input module

Jasco Fourier Transform IR Spectrometer, FT-IR model 410

  • SPECAC Silver Gate Single Reflection Germanium ATR System Optics

Vacuum Desiccating Cabinet Fisher Isotemp model 201

  • SPCPOF110 Rotary Vein Vacuum Source

Beckman UV Spectrophotometer

  • DU-7, w/Epson 9-dot matrix printer

Shimadzu UV Spectrophotometer UV2401 PC

  • w/Isotemp variable temperature unit Fisher Isotemp model 10165
  • w/computer control, HP LaserJet 1100

Shimadzu Fluorospectrophotometer RF-1501

  • w/computer control, HP LaserJet 1100

Isco Combiflash Preparative Low-pressure Liquid Chromatograph (LPLC)

  • Integrated fraction collector, 6-port injection valve

Tokyo Rikakikai (Eyela) Droplet Countercurrent Chromatograph (DCCC) model DCC-A

  • Integrated mobile/stationary phase selection and 6-port injection valves
  • Waters series 600 liquid pump

PC Inc MLCCC "Ito Apparatus"

  • 300 ml capacity, single coil instrument
  • Waters series 600 liquid pump
  • 6-port valve injection system

Dionex biocompatible HPLC LC20 (needs to be set-up)

  • with gradient pump GP40 and
  • electrochemical detector ED40
  • control computer and Dionex software

HP Gas Chromatograph model 5890 (needs to be set-up)

  • with model 7673 autosampler and integrator